| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 321 | Holdemania filiformis | AAATGGCTGCGGACGTTG | GCGTCGGAAACCTGACAGA | 59.05 | 60.01 | 281 | 71 |
100.00%
|
50.00%
|
| Primer 322 | Holdemania filiformis | TGCCTGGTGTGCGGAAAT | AAGGAAGCGTCGGCAAGA | 59.88 | 59.26 | 246 | 71 |
100.00%
|
50.00%
|
| Primer 323 | Holdemania filiformis | ATTTCCTGCAGGCCAGCT | TGCGTGATCGTAATGCCGA | 59.23 | 59.86 | 199 | 71 |
100.00%
|
50.00%
|
| Primer 324 | Holdemania filiformis | AAGTCGCAACGGTTCTGC | GCCTAACTTGGTGTGCGGA | 58.67 | 60.30 | 254 | 71 |
100.00%
|
50.00%
|
| Primer 325 | Holdemania filiformis | GCTTCGCAACGCACATGA | AGCCGATTGACGTGCAGA | 59.44 | 59.34 | 211 | 71 |
100.00%
|
50.00%
|
| Primer 326 | Holdemania filiformis | GCTTCGCAACGCACATGA | AAGCCGATTGACGTGCAGA | 59.44 | 60.01 | 212 | 71 |
100.00%
|
50.00%
|
| Primer 327 | Holdemania filiformis | GGCCAAAACCGCAAGCAA | AGATCCACAGCCTGAACGC | 59.89 | 60.08 | 198 | 71 |
100.00%
|
50.00%
|
| Primer 328 | Holdemania filiformis | TGAAACGACGATCGGCTGT | ACTCATGCTTGTCCTCCGC | 59.71 | 60.08 | 191 | 71 |
100.00%
|
50.00%
|
| Primer 329 | Holdemania filiformis | AGGTCACGACGATCCTGGA | TCATCGCGACGCTGTTGT | 60.00 | 60.05 | 213 | 71 |
100.00%
|
50.00%
|
| Primer 330 | Holdemania filiformis | CCGTCGGGTTCGTCACAAT | ATCGAGTCGATCCGTTCGC | 60.37 | 60.01 | 235 | 71 |
100.00%
|
50.00%
|